Kotoko lost to Samartex in a rescheduled game from Monday to Tuesday at the Baba Yara Sports Stadium.
The Porcupine Warriors were in the hunt to keep up places with the title leaders, GoldStars and Medeama SC.
The first saw few chances created, but it was the goalkeeper, Aziz Haruna Dari, who was the busiest of the two goalkeepers, pulling brilliant saves from Christian Boateng and Latif Abdul shots.
The first half failed to produce any goals between the two teams as it rained off and was played on Tuesday at 9 :00 am.
Coach Prince Owusu pulled out Inusah Adam and Baba Yahaya for the more attacking duo Dogo and Donzo.
Christian Boateng put his side into the lead in the 56th minute.
Emmanuel Mamah doubled the score with eight minutes to end proceedings.
There was late drama as Samed Kyei received a second bookable offence in the 85th minute.
Agbesimah was deemed to have fouled Albert Amoah in the box, with referee Nathan Anafo awarding a penalty.
Samba O’neil missed the penalty, but Albert Amoah scored from the rebound to peg the game at 2-1.
Kotoko will travel away to Medeama SC, while Samartex will be at home to Berekum Chelsea.
