Thiago has been ruled out of Spain’s final World Cup qualifier away to Israel on Monday after spraining his ankle.
The Bayern Munich midfielder played the full 90 minutes and was amongst the scorers as Spain beat Albania 3-0 last night to secure their place in the World Cup finals.
However, Thiago has now withdrawn from the squad and will return to Bayern for treatment.
Barcelona defender Gerard Pique and Manchester City midfielder David Silva will also miss the Israel game due to injuries.
A statement from the Spanish Football Federation read: “International Thiago Alcantara (Bayern Munich) leaves the concentration of the national team since he suffered a sprained ankle that will prevent him to play against Israel.
“This morning the player was given an MRI scan that determined the extent of the injury.
“The medical services of the RFEF have already contacted the members of their club to send them the medical report.
“The Spanish national team, after qualifying for the 2018 FIFA World Cup, will travel to Israel with 22 players, following injuries from Thiago and Gerard Pique (FC Barcelona) and David Silva (Manchester City) both for sanction.”
Given that they have already qualified, Thiago’s absence isn’t a major problem for Spain and will allow other members of Julen Loepetegui’s side to receive playing time.
Bayern, who have endured a difficult start to the season, will hope that Thiago isn’t sidelined for a lengthy period.
So far this campaign Thiago has retained possession very well – he has been successful with 91 per cent of his attempted passes in the Bundesliga – while he has created seven chances.